Your post is unclear. Are you self employed or a PAYE worker? Or both?
If the PAYE job is completely separate from the company that you and your wife are involved in/own then your Jobseekers Benefit would not be affected, and any calculation would be based on your PAYE wages. If however, you are trying to claim as a PAYE worker in your wife's company, it is very possible that you will not be considered to be a genuine PAYE worker and will have no entitlement to Jobseekers Benefit.