Yes, most bank staff in a branch or business centre would have access to an account. However depending on her role, unless she is involved in lending she might have had no part in the decision making process and if there was something wrong with your accounts, I'm sure your relationship manager or lending manager would have found out anyway, after all, that is their job
If you still feel something is amiss, you should ask your relationship manager why the loan was refused. If you are still not satisfied after that then you should make a complaint in writing. Most system access in banks is password controlled so there should be an electronic audit trail of who looked at what in the bank